Current Issue : January-March Volume : 2023 Issue Number : 1 Articles : 6 Articles
Chronic cerebral hypoperfusion (CCH) is a cardinal risk factor for Parkinson’s disease dementia (PDD), but this potential causality lacks mechanistic evidence. We selected bilateral common carotid artery occlusion (BCCAO) to simulate chronic cerebral hypoperfusion in the rat model of PD induced by typical neurotoxin 6-hydroxy dopamine (6-OHDA). Four weeks after unilateral injection of 6-OHDA into the medial forebrain bundle, rats underwent BCCAO. Male Sprague-Dawley rats were divided into five groups of ten, including sham, PD+BCCAO 2 weeks, PD+BCCAO 1 week, PD, and BCCAO 2 weeks. Then, open field test (OFT) and Morris water maze test (MWM) were used to assess the PDD-like symptoms in rats. Also, the pathological manifestations and mechanisms of BCCAO impairing cognitive functions have been explored via hematoxylineosin staining, Nissl staining, immunohistochemistry, immunofluorescence, RNA sequencing analysis, lipidomics, and quantitative real-time polymerase chain reaction. In this study, we found that CCH could aggravate PDD-like cognitive symptoms (i.e., learning memory and spatial cognition) and PDD-like pathology (higher expression of α-Syn and Aβ in prefrontal cortex and striatum). Moreover, a potential relationship between differentially expressed mRNAs and lipid metabolism was revealed by RNA sequencing analysis. Lipidomics showed that CCH could affect the intensity of 5 lipids, including sphingomyelin (SM 9:0;2O/26:2; SM 8:1;2O/25:0; and SM 8:0;2O/28:4), cardiolipin, lysophosphatidylcholine, cholesteryl ester, and triacylglycerol. Interestingly, the KEGG pathway analysis of both RNA sequencing analysis and lipidomics suggested that CCH leaded to learning impairment by affecting sphingolipid metabolism. Finally, we found that CCH disrupts the sphingolipid metabolism by affecting the mRNA expression of SMPD1 and SMS2, leading to the accumulation of sphingomyelin in the prefrontal cortex. In summary, CCH, an independent exacerbating reason for impairment in learning and memory within the pathopoiesis of PD, aggravates Parkinson’s disease dementia-like symptoms and pathology in 6-OHDA-lesioned rat through interfering with sphingolipid metabolism....
Objective: To describe the radiological and pathological presentation of a salivary heterotopia in clivus. The lesion was detected incidentally in a 57-year-old woman. Material and Methods: Radiologic imaging (CT scan and MRI) and histological picture obtained from a clival lesion. Results: Radiologically the lesion presented as a well-limited lytic lesion with a diameter of 1.5 cm and accompanied by a cerebrospinal fluid secondary fistula. Histological examination revealed non-pathological salivary gland tissue. Conclusion: The differential diagnosis of this rare entity mandates a histological examination of the lesion....
Background. Vertical transmission of several viruses during pregnancy has been shown to cause adverse fetal outcomes. The question about the possibility of a similar outcome in association with SARS-CoV-2 has been raised in recently published articles. Indeed, the rate of transmission through the placenta to the fetus reported in women with COVID-19 has been shown to form a minority. The aim of this study was to explore the possible histopathological changes in the placenta of pregnant women with COVID-19 after delivery and those changes in the umbilical cord. Methods. A case-control study including a total of 50 full-term pregnant women with COVID-19 and 60 control pregnant females. Histopathological evaluation of placental tissues and umbilical cords were reported. Results. The main findings in the umbilical cord were increased thickness of vessels, thrombus formation, endothelins, and narrow lumen; except for the increased thickness of blood vessels, these findings were more frequently seen in women with COVID-19, in comparison with control women in a significant manner (p < 0:05). Increased thickness of blood vessels was more significantly observed in the control group compared to the COVID-19 group (p < 0:01). Findings of the placenta included avascular villi, fibrin, thrombosis, and meconium macrophage in various combinations. Except for fibrin as the sole findings, all other findings including combinations were more frequently encountered in the study group in comparison to the control group (p < 0:05). Conclusion. Pregnant women with COVID-19 have significant pathological alterations in the placenta and umbilical cord. These findings reflect the capability of SARS-CoV- 2 in causing immunological reactions to the placenta, either directly or indirectly, and these pathologies may be linked to the higher rate of adverse neonatal outcomes and maternal admission to the intensive care unit....
In this article, in order to explore the application of a diagnosis system for lung cancer, we use an auxiliary diagnostic system to predict and diagnose the good and evil attributes of chest CT pulmonary nodules. This research improves the new diagnosis method based on the convolutional neural network (CNN) and the recurrent neural network (RNN) and combines the dual effects of the two algorithms to process the classification of benign and malignant nodules. By collecting H-E-stained pathological slices of 652 patients’ lung lesions from two hospitals between January 2018 and January 2019, the output results of the improved 3D U-net system and the consistent results of two-person reading were compared. This article analyzes the sensitivity, specificity, positive flammability rate, and negative flammability rate of different lung nodule detection methods. In addition, the artificial intelligence system’s and the radiologist’s judgment results of benign and malignant pulmonary nodules are used to draw ROC curves for further analysis. The improved model has an accuracy rate of 92.3% for predicting malignant lung nodules and an accuracy rate of 82.8% for benign lung nodules. The new diagnostic method using the convolutional neural network and the recurrent neural network can be very effective for improving the accuracy of predicting lung cancer diagnosis. It can play a very effective role in the disease prediction of lung cancer patients, thereby improving the treatment effect....
Context: Childhood eye health is a real public health problem in our context, the consequences of which go beyond vision, affecting education, social participation and future economic productivity. The aim was to study the frequency of ocular surgical pathologies in children aged 0 to 15 years. Patients and Methodius: This study covered a 4 years period from January 1, 2016 to December 31, 2020. It was a retrospective study of a descriptive and analytical nature. It concerned all children aged 0 to 15 who had undergone surgery for ocular pathology during the study period and who were included in the operating room register. Results: Out of 4974 patients of all ages operated for ocular surgical pathology, 124 were children aged 0 to 15 years or 2.49%. The 9 - 12 age group was the most represented with 29.03%; the mean age was 7.3 years ± 4.6 with extremes of 3 months and 15 years. A male predominance was observed at 62.90%. Pathologies of the lens, cataract and eyelids, traumatic edema represented the most frequent eye conditions in our patients with respectively 51 cases or 41.13% in the right eye (OD), 54 cases or 53.55% in the left eye (OG) and 14 cases or 11.29% in OD and OG followed by endemic limboconjunctivitis of the tropics (LCET), 10 cases or 3.23%, in OD and hemorrhage under conjunctival 13 cases or 10.48% in OG. Cataract surgery with the Technique Manuel Small Incision Cataract Surgery + Implantation in the posterior chamber (MSICS + ICP) was the most used in 74% of cases. General anesthesia was the most widely used anesthetic method in 100% of cases. The clinical course was favorable at 45.97%. Conclusion: We note a relatively low hospital frequency of ocular surgical pathologies in children, with a male predominance. The early consultation period and the im-provement of the technical platform could improve their management....
Objective: We studied the prognosis and clinicopathological background of lung adenocarcinoma predominance among patients who underwent lobectomy using data from the Japanese Joint Committee of Lung Cancer Registry. Methods: Two thousand eight hundred sixty-three cases were extracted. Recurrence free survival (RFS) rates, overall survival (OS) rates and clinicopathological factors and epidermal growth factor receptor (EGFR) mutation status were examined. Results: Median follow-up period was 65.5 months. Adenocarcinoma predominance was sub-grouped according to OS and RFS rate. In pathological stage I, 5-year RFS and OS rates were respectively 92.2% and 95.8% in group A (adenocarcinoma-in-situ + minimally invasive adenocarcinoma), 89.3% and 92.1% in group B (lepidic), 79.2% and 89.7% in group C (papillary + acinar + variants) and 69.0% and 79.0% in group D (solid + micropapillary). In pathological stage II + IIIA, they were, 43.6% and 72.4% in B, 39.5% and 66.9% in C and 31.0% and 53.7% in D. Group D showed significant worst outcome both in stage I and II + IIIA. Up stage rate from clinical stage I to pathological stage II + IIIA was 0.0%, 3.7%, 15.9% and 33.3%. The frequency of lymph-vessel, vascular, pleura invasion and positive EGFR mutation were 0.0%, 0.0%, 0.0% and 57.1% in group A, 15.6%, 10.0%, 12.1% and 55.1% in B, 36.6%, 31.8%, 29.7% and 44.9% in C, 50.2%, 57.8%, 38.9% and 21.3% in D. In group D, lymph-vessel, vascular and pleura invasion were most, EGFR mutation was least frequent not only in pathological stage I but also stage II + IIIA. In multivariate analysis, age, pathological stage, vascular invasion, and group D were independent factors affected RFS and OS. Conclusion: Limited to lobectomy cases, solid + micropapillary was independent prognostic factor both in early and locally advanced stage. Its malignant degree was related to the frequency of pathological invasive factors and EGFR mutation status....
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